Biochem/physiol Actions

Target Sub-Family: CMGC

Protein Target: CDK1/cyclinB

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: expressed individually by baculovirus in Sf21 insect cells

C-terminal His6-tagged human full length cdk1, and N-terminal GST-tagged human full length cyclin B

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sepharose is a registered trademark of Cytiva

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-450M when ordering the 250 µg size.

Physical form

Purified using Ni²⁺/NTA-agarose and GST-agarose, respectively. The cdk1 is then activated using CAK and repurified by Q Sepharose^® and Ni2+/NTA-agarose

Quality

routinely evaluated by phosphorylation of the Histone H1 substrate

Storage and Stability

6 months at -70°C